Tnfα and il-1β modify the mirna cargo of astrocyte shed extracellular vesicles to regulate neurotrophic signaling in neurons

HIGHLIGHTS

• who: Amrita Datta Chaudhuri from the Primary hippocampal and cortical neurons were prepared from day , embryos of Sprague-Dawley rats as described previously In brief, hippocampal tissues were separately dissociated by gentle trituration in a calciumfree Hank's balanced salt solution and centrifuged at, ×g. Cells were resuspended in Neurobasal media (Gibco) containing , supplement (Thermo Fisher Scientific), % antibiotic/antimitotic solution (, Unit of penicillin G/ml, mg streptomycin/ml and , μg amphotericin B/ml) (Sigma), and plated at a density of, cells/ml in, well plates (Corning) coated with polyethyleneimine (Sigma) or, cells/ml in multichannel electrode array . . .
  

   

  

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