HIGHLIGHTS
- who: Miguel M. u00c1lvarez from the (UNIVERSITY) have published the research: TP53-dependent toxicity of CRISPR/Cas9 cuts is differential across genomic loci and can confound genetic screening, in the Journal: (JOURNAL) of 20/Dec/2021
- what: By analyzing isogenic cell line pair CRISPR experiments jointly with previous screening data from across ~900 cell lines, the authors show that TP53-associated break toxicity is higher in genomic regions that harbor active chromatin, such as gene regulatory elements or transcription elongation histone marks. Because pooling data across multiple sgRNA loci in a gene could obscure differences . . .
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