Transcription factor nkx2-1 drives serine and glycine synthesis addiction in cancer

HIGHLIGHTS

• who: Elien Heylen from the Cell culture , (ACC, ), Ba/, (ACC, ), (ACC, ), (ACC, ) and T (ACC, ) cell lines were obtained from the German Collection of Microorganisms and Cell Cultures GmbH (DSMZ)NCI-, were historically obtained from Molecular Biology Dept. at Maastricht University (P14). , and T cells were cultured in DMEM (Gibco) with, % foetal calf serum (FCS) (Gibco). For assays in serine/glycine-depleted condition, cells were plated in DMEM without serine and glycine (US Biological life Sciences, ), supplemented with, ., g/L glucose (Sigma-Aldrich), ., g/L sodium bicarbonate (Sigma-Aldrich), GlutaMAXu2122 (Gibco) and, % dialysed serum (Life Technologies, ). , and . . .
  

   

  

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