HIGHLIGHTS
- who: Clostridium autoethanogenum and colleagues from the C The Author(s) Published by Oxford University PressQueensland, Australia have published the Article: Transcriptional control of Clostridium autoethanogenum using CRISPRi, in the Journal: (JOURNAL)
- what: We found that sgRNA targeting regions proximal ( 100 bp) to the start codon with were most effective at generating strong KD phenotypes in C. autoethanogenum.
- how: The authors applied CRISPRi to KD expression of three genes of interest-a secondary alcohol dehydrogenase (sADH) alpha-acetolactate decarboxylase (BudA) and a TetR family transcriptional regulator (TetR). sADH and BudA are pivotal for . . .
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