Transcriptional suppression of connexin43 by tbx18 undermines cell-cell electrical coupling in postnatal cardiomyocytes

HIGHLIGHTS

• who: Nidhi Kapoor from the (Invitrogen)Western blots were performed using specific antibodies against Cx, (Sigma, ), Cx, (Invitrogen, ), and Cx, (a generous gift provided by Thomas H. Steinberg, Washington University, St. Louis). Briefly, AdTBX18- and Ad-GFP-transduced NRCMS were homogenized in RIPA buffer containing a protease inhibitor mixture (Sigma). Protein content was quantified by BCA assay, and cell lysates (, ␮g per lane) were run on a, % SDS-polyacrylamide gel and transferred onto a PVDF membrane. Then the transferred membrane was incubated with a primary antibody overnight at , °C, followed by a, h incubation with a peroxidase . . .
  

   

  

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