Transcriptome profiling of mouse samples using nanopore sequencing of cdna and rna molecules

HIGHLIGHTS

  • who: Camille Sessegolo from the CNRS have published the Article: Transcriptome profiling of mouse samples using nanopore sequencing of cDNA and RNA molecules, in the Journal: Scientific Reports Scientific Reports

SUMMARY

    The authors follow the protocols recommended by the manufacturers to generate the three following datasets on each tissue: Illumina cDNA-Seq, Nanopore cDNA-Seq and Nanopore RNA-Seq In fact ONT RNA-Seq reads are strand-specific which is of high value for the alignment and splice site detection. But even though this horizontal coverage is quite high, the proportion of reads . . .

     

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