Transcriptomic and enzymatic analysis reveals the roles of glutamate dehydrogenase in corynebacterium glutamicum

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  • who: Fanglan Ge from the Details on the bacterial strains and plasmids which were used in this study are listed in the Table, while oligonucleotide sequences are presented in Additional file, : Table S Escherichia coli strains DH5u03b1 and , (DE3) were stored in the laboratory, and used for gene cloning and expressing purposes, respectively. Corynebacterium glutamicum strain , (Zheng et_al, ), gifted by Professor Pu Zheng of Jiangnan University, was used as the parent strain. The plasmid pK18mobsacB (Schu00e4fer et al., ) was used for gene disruption in strain, . Plasmid p, was used to over-express genes in C.glutamicum. LBHIS . . .

     

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