HIGHLIGHTS
- What: This study provides valuable insights into the genetic basis of complex traits such as yield and resilience to biotic and abiotic stress. This approach has been effective for crops such as maize, tomato, sorghum, chickpea, rice, and wheat. This approach provides a nontransgenic approach, avoiding the introduction of foreign genes into the genome, and enables high-throughput screening of large populations for mutations efficiently. The primary motivation behind the development of HE was to overcome the limitations associated with traditional genome editing techniques, such as CRISPR‒Cas9, which rely on DSBs to make genetic modifications (Wang . . .

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