Tsp50 promotes the warburg effect and hepatocyte proliferation via regulating pkm2 acetylation

HIGHLIGHTS

• who: Feng Gao from the Memorial Institute , medium supplemented with, % fetal bovine serum (FBS)Huh, cells and Bel, cells were cultured in RPMI , medium with, % FBS. The HEK- T cells were cultured in, DMEM medium supplemented with, %FBS. All mediums were supplemented with , units/ml penicillin and , mg/ml streptomycin. Cells were maintained at , °C in incubator containing, % CO2. When the cells confluence reached, %, µl RPMI , serum-free medium was mixed with , µl of, tremeGENE HP and , µg plasmid for transfection. The primers for plasmid construction were shown in Supplementary Table, . qRT-PCR detection and Western blot . . .
  

   

  

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