Tumor cells-derived conditioned medium induced pro-tumoral phenotypes in macrophages through calcium-nuclear factor κb interaction

HIGHLIGHTS

  • who: Yuexin Zhang from the Institutional Animal Care and Use Committee of Sichuan University (Chengdu, Sichuan, China)All the experiments were conducted in compliance with the protocols approved by the Institutional Animal Care and Use Committee of Sichuan University (Chengdu, Sichuan, China). Our study was conducted in accordance with ARRIVE guidelines. For each experiment, mice were anaesthetized using isoflurane and sacrificed. Three or four mice were euthanasia to isolate bilateral tibias and femurs in sterile condition. Bone marrow was flushed out by , medium (C BT, Gibco). The bone marrow was centrifuged and resuspended with red blood cell . . .

     

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