Tuning heterologous glucan biosynthesis in yeast to understand and exploit plant starch diversity

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  • who: Barbara Pfister from the The genotypes of Saccharomyces cerevisiae (yeast) strains used in this study are presented in Additional file, sheet All strains derive from CEN.PK113- C (MATa MAL2- , his D ura3-52), kindly provided by Prof. Barbara A. Halkier (University of Copenhagen, Denmark) and named wild type here. Yeast strains were generated by integrating constructs at gene-encoding loci, thereby deleting them, or at the intergenic loci , and , on chromosome , [72]. Yeast strains , and , are described in [25]. Yeast transformation was essentially conducted as described [72]. Correct construct integration in streakpurified transformants was tested . . .

     

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