Tuning instability of non-columnar neurons in the salt-and-pepper whisker map in somatosensory cortex

HIGHLIGHTS

• who: Han Chin Wang from the Surgery and viral injection Mice (25, months old) were anesthetized with isoflurane (1-1.5% in, ), and a stainless-steel head holder with , mm aperture was affixed to the skull using cyanoacrylate glue and dental cement., and , whisker columns were localized using transcranial intrinsic signal optical imaging61, . A , mm diameter craniotomy was made centered on the , column. For viral GCaMP6s expression, AAV1-Syn-Flex-GCaMP6s-WPRE-, (Addgene #100845-AAV1) was injected at, locations surrounding the , column at , u00b5m and , u00b5m depth. A chronic cranial window (, mm diameter glass coverslip, #, thickness, CS . . .
  

   

  

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