HIGHLIGHTS
- who: Yige Li from the NSCs were taken from the left and right hippocampus of , days old mouse embryos, at a time when these cells are highly proliferate, followed the guidelines and agreements approved by the Animal Care and Use Committee of Southeast UniversityBriefly, blood vessels and meninges on the hippocampus were removed in phosphate-buffered saline (PBS) at , u00b0C. Then PBS was fully removed and Accutase solution (Thermo, USA) was added to digest the hippocampus in a, % u00ad, incubator at , u00b0C for , min. After Accutase solution was fully washed, DMEM-, medium containing, %, % Penicillin-Streptomycin, ng/mL . . .
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