Two isoforms of a divalent metal transporter (dmt1) in schistosoma mansoni suggest a surface-associated pathway for iron absorption in schistosomes*

HIGHLIGHTS

• who: Danielle J. Smyth from the For immunofluorescence, unfixed adult Smansoni worms were snap-frozen in Cryomatrix (ThermoShandon) for cryostat sectioning. All incubations used, % skim milk powder in phosphate-buffered saline as incubation buffer. Cryosections were incubated with Sm, serum (1:20) or with the heterologous antiserum diluted (1:, in incubation buffer) followed by goat anti-rabbit secondary antibody conjugated to Cy, fluorophore (Jackson ImmunoResearch Laboratories) (1:, in incubation buffer). Negative controls were as described for Western blotting (above). Sections were examined and photographed using a Leica DM IRB fluorescence microscope equipped with a Leica DC , digital . . .
  

   

  

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