HIGHLIGHTS
SUMMARY
To inhibit the amplification of WT gene copy or delay the amplification beyond 50 cycles, which would avoid overlap with amplification of low copies of mutant, the authors tested SuperSelective primers 18-14/21-6:1:0 (inh15_SSP2) with increased lengths of the intervening_sequence (from 14 to 21 bp) (forming an asymmetric bubble) in RT-PCR assays. The authors tested different bridge-intervening_sequence length combinations-20/8, 8/8, and 8/20 (inh15_SSP7-9)-in the SuperSelective primer designs. SuperSelective primers with a long bridge but short intervening_sequence 18-20/8-7:1:0 . . .
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