Unravelling the first key steps in equine herpesvirus type 5 (ehv5) pathogenesis using ex vivo and in vitro equine models

HIGHLIGHTS

• who: Jolien Van Cleemput from the Equine PBMC were isolated as described previously [31]The collection of blood was approved by the ethical committee of Ghent University (EC2017/118). Ten hours post seeding, ­CD172a+ monocytic cells had adhered to the plastic (purity >, %, as assessed by flow cytometry [31]) and non-adherent cells consisted of two dominant leukocyte populations: T and B lymphocytes. Following removal of non-adherent cells, equine ­CD172a+ monocytes were further maintained in RPMI supplemented with, % fetal calf serum (FCS) and antibiotics. Equine T lymphocytes were separated from B lymphocytes by negative selection magnetic-activated . . .
  

   

  

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