Upregulation of tgf-β-induced hsp27 by hsp90 inhibitors in osteoblasts

HIGHLIGHTS

• who: Gen Kuroyanagi from the Cloned osteoblastic MC, cells were generously provided by DrM. Kumegawa (Meikai University, Sakado, Japan). The osteoblastic MC, cells, established from neonatal mouse calvaria [24], were incubated at , u00b0C with, % u00ad, and cultured in u03b1-minimum essential medium (u03b1-MEM) supplemented with, % fetal bovine serum (FBS) as previously described [25]. Cells were seeded into, mm diameter dishes (, u00d7 u00ad, cells/dish) in u03b1-MEM supplemented with, % FBS. u03b1-MEM medium was supplemented with, .3% FBS after , days. After , hours, the cells were used for the experiments. Cells of passages under , were selected for . . .
  

   

  

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