Using exosomal mirnas extracted from porcine follicular fluid to investigate their role in oocyte development

HIGHLIGHTS

• who: Junhe Hu from the All experimental porcine ovaries were collected from a local abattoir (usually , porcine ovaries each time collected from QinYangMuYe Limited Responsibility Company, LouDi city, HuNan Province, China) and transported to our lab within , h of slaughter in a thermo-flask containing penicillin and streptomycinAll experiments were conducted in accordance with the Institutional Ethics Committee of Hunan University of Humanities, Science and Technology. Ovaries were washed at least three times with physiological saline solution and porcine follicular fluid was collected by separately aspirating with a, gauge needle attached to a , mL syringe from small . . .
  

   

  

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