Variable expression and silencing of crispr-cas9 targeted transgenes identifies the aavs1 locus as not an

HIGHLIGHTS

• who: Jamie R. Bhagwan and colleagues from the Department of Stem Cells, Tissue Engineering and Modelling, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, RD, UK have published the research work: Variable expression and silencing of CRISPR-Cas9 targeted transgenes identifies the AAVS1 locus as not an, in the Journal: (JOURNAL) of 10/07/2020
• what: The authors show variable success targeting the AAVS1 locus with the genetically encoded calcium indicator R-GECO1.0 or a doxycycline-inducible HOXA9-T2A-mScarlet cassette using a CRISPR Cas9 nickase approach. With the use of the AAVS1-targeted . . .
  

   

  

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