Vascular mimicry induced by m6a mediated igfl2-as1/ar axis contributes to pazopanib resistance in clear cell renal cell carcinoma

HIGHLIGHTS

• who: Bo Cheng from the Immunohistochemical staining (IHC) The deparaffinized ccRCC sections (informed consent was obtained from patients and experiments were performed with the approval of the Ethics Committee of the Affiliated Hospital of Southwest Medical University) or cell line derived tumor sections were treated with methanol containing, % peroxidase for , min and followed by antigen retrieval in citrate buffer (pH=60) at , u00b0C for , min. Sections were then blocked by, % BSA +, % milk PBS for , h at room temperature and followed by incubation with antiAR (1:100, Santa Cruz) or Ki, (ab15580, abcam) at , u00b0C overnight. Then biotin . . .
  

   

  

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