HIGHLIGHTS
- who: Zhiwei Liao from the The human primary NP cells were isolated from patients who underwent surgery for disc excisionThe experimental design and protocols were endorsed by the Ethics Committee of Tongji Medical College, Huazhong University of Science and Technology. Informed consent was obtained from all involved patients. Briefly, the NP tissues were collected and digested with, .2% collagenase II for , h. After washed and centrifuged, the precipitation was resuspended and cultured in Dulbecco`s Modified Eagle Medium/Nutrient Mixture , (DMEM/F-12) containing, % fetal bovine serum (HYcezmbio, Wuhan, China). For MSCs isolation, human bone marrow specimens . . .
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