Vesicle mir-195 derived from endothelial cells inhibits expression of serotonin transporter in vessel smooth muscle cells

HIGHLIGHTS

• who: Junzhong Gu from the Cell culture and transfectionHuman coronary and pulmonary smooth muscle cells (HCASMCs and HPASMCs) were purchased from Promo Cell. Human umbilical vein endothelial cells (HUVEC) and human umbilical vein smooth muscle cells (HUVSMCs) were freshly isolated from human umbilical cord vein as previously described38, and cultured with ECs growth medium (Promo Cell) and DMEM/, supplemented with, % FCS, % sodium pyruvate, % glutamine and, % penicillin and streptomycin respectively. The protocols for human umbilical cord collection and isolation of human umbilical vein endothelial cells (HUVECs) and human umbilical vein smooth muscle cells (HUVSMCs) were approved by University . . .
  

   

  

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