Vesicular polyamine transporter mediates vesicular storage and release of polyamine from mast cells*

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  • who: Tomoya Takeuchi from the Preparation of BMMCs and RPMCs-Male, week-old BALB/c mice and male, week-old Wistar/ST rats were obtained from Japan SLC (Shizuoka, Japan)All animal procedures and care were approved by the institutional animal care and use committee and were carried out according to the guidelines of Okayama University. BMMCs were prepared as described previously (35). Briefly, bone marrow cells were isolated from BALB/c mice. These cells were cultured in RPMI , medium supplemented with, % heat-inactivated FBS, units/ml penicillin, units/ml streptomycin, ␮M, mercaptoethanol, ., mM non-essential amino . . .

     

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