Wisp1/ccn4 inhibits adipocyte differentiation through repression of pparγ activity

HIGHLIGHTS

• who: Nathalie Ferrand from the The entire open reading frame of , cDNA obtained from the genomic DNA of , cells was inserted into the pCEP4-Flag vector (Invitrogen) as described, and referred to as pCEP4-Flag-WISP Small interfering RNA (siRNA) oligonucleotides for , were a pool of , target-specific, nt siRNAs designed to knock-down gene expression (Santa Cruz Biotechnology). Control siRNA (Santa Cruz Biotechnology) was used as a negative control for evaluating RNAi off-target effects. Plasmids for PPRE-TK-Luc, mPPRE-TK-Luc, UAS-TK-Luc, Gal4-PPARγ-LBD and PPARγ have been described previously, and . . .
  

   

  

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