Yeast-based directed-evolution for high-throughput structural stabilization of g protein-coupled receptors (gpcrs)

HIGHLIGHTS

• who: M. Meltzer from the DepartmentGurion University have published the research: Yeast-based directed-evolution for high-throughput structural stabilization of G protein-coupled receptors (GPCRs), in the Journal: Scientific Reports Scientific Reports
• what: In contrast to the previous studies focused on A2aR ­thermostability19,20, the authors used the resistance to short-chain detergents-a prerequisite for successful vapor diffusion crystallization-as a primary criterion for clone selection.
• how: The authors therefore assume that a much milder treatment with 1% OG for 1 min which the authors used for yeast wall permeabilization does . . .
  

   

  

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