Yellow water lilies (nuphar, nymphaeaceae) in great britain: a new hybrid, a reappraisal of records, and a revised status of n. advena

HIGHLIGHTS

• who: R. V. Lansdown and M. Ruhsam from the Genetics Following the manufacturer`s protocol, the DNeasy Plant kit (Qiagen, Hilden, Germany) was used to extract DNA from collected leaf samples stored in silica gelThe nuclear DNA-barcoding marker internal transcribed spacer , (ITS2, Hollingsworth et_al, ) was used to distinguish between Nuphar lutea and N. advena. Downloaded sequences of the available , sequences for both species from the National Center for Biotechnology Information (GenBank, https://www.ncbi.nlm.nih.gov/nuccore/) showed that the two species differed by nine single nucleotide polymorphisms (SNPs) along abp stretch of ITS2. The . . .
  

   

  

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