Zika virus-induced tnf-α signaling dysregulates expression of neurologic genes associated with psychiatric disorders

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  • who: Po-Lun Kung from the ZIKV strain , was originally provided by DrAndrew Oberst (University of Washington, Seattle, WA, USA). ZIKV-DAKAR-MA was first generated [7] and generously provided by Dr. Michael Diamond (Washington University, St. Louis, MO, USA). Viral stocks were generated by infecting Vero cells (MOI, .01) and harvesting supernatants at, hpi. Viral titers of stocks were determined via plaque assay on Vero cells (ATCC, #CCL-81). Cells were maintained in DMEM (Corning #10-013-CV) supplemented with, % Heat Inactivated FBS (Gemini Biosciences #100106), % Penicillin-Streptomycin-Glutamine (Gemini Biosciences #400-110), % Amphotericin B (Gemini . . .

     

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