153 cas9-expressing cattle using all-in-one crispr/cas9 for bovine genome editing

HIGHLIGHTS

• What: The authors hypothesized that if cattle with CRISPR/Cas9, including the reporter fluorescence gene, FatI, or single-guide RNA (sgRNA) for PRNP were born and the germline transmission confirmed, the resulting transgenic cattle would be a powerful resource for a variety of applications in the bovine genome editing field. Application of Cas9-expressing somatic_cells in gene engineering Since cattle with confirmed Cas9 expression in the primary somatic_cells also had Cas9 transgene integration, an analysis was conducted to determine whether the presence of specific sgRNAs targeting given genes could induce mutations in those target genes. The aim . . .
  

   

  

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