Vibrio cholerae rbmb is an α-1,4-polysaccharide lyase with biofilm-disrupting activity against vibrio polysaccharide (vps)

HIGHLIGHTS

• What: Using a fluorescence-based biochemical assay the authors show that RbmB specifically cleaves VPS in vitro under physiological conditions. The authors demonstrate that the product of the cleavage contains a double bond in the modified guluronic acid ring strongly suggesting that RbmB is cleaving using a glycoside lyase mechanism. The authors show that recombinant RbmB from V. cholerae and the related aquatic species Vibrio coralliilyticus are both able to disrupt living V. cholerae biofilms. Using a combination of biochemical and analytical tools the authors show that RbmB indeed cleaves VPS into tetra- and octasaccharide fragments at . . .
  

   

  

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