Development of a copro-rpa-crispr/cas12a assay to detect echinococcus granulosus nucleic acids isolated from canine feces using naoh-based dna extraction method

HIGHLIGHTS

  • What: In this study, a comparative analysis was conducted between RPA-CRISPR/Cas12a or RPACRISPR/Cas12a-NaOH and two enzyme-linked immunosorbent assay (ELISA) kits, along with a quantitative polymerase_chain_reaction (qPCR) method. The study provides support for the efficacy of the RPA-CRISPR/Cas12a or RPA-CRISPR/ Cas12a-NaOH assay to detect E. granulosus in canine feces, demonstrated by their satisfactory LOD, specificity, and sensitivity.
  • Who: Guoqing Shao and colleagues from the Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, PRChina have published the research work: Development of a Copro-RPA-CRISPR . . .

     

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