HIGHLIGHTS
- What: The results of the analysis showed that 41 sperm proteins were found in the 2-year age group, 45 sperm proteins in the 4-year age group, and 41 sperm proteins were found in the age group of more than 10 years. In this study, the expression of sperm proteins in different age groups of Simmental bulls and their relevance to sperm functions and fertility were examined. Ethical approval was not required for the studies on animals in accordance with the local legislation and institutional requirements because only commercially available established cell lines were used.

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