SUMMARY
Cell viability assay BT474, T47D, MCF-7, MDA-MB-231, MDA-MB-468, HEK293, HEK293/EGFR, A431, MDA-MB-231-shEGFR, MDA-MB-468-shEGFR, MDA-MB-231-shEGFR + EGFR, MDA-MB-468-shEGFR + EGFR, MCF-7/EGFR cells were seeded and incubated with different concentrations of cantharidin for 48 h. The authors then carried out the lentivector transfection to stably downregulate EGFR in high EGFR-expressing MDA-MB-231 and MDA-MB-468 cells, as well as further restoring EGFR levels. MTT and flow cytometry results showed that EGFR knockdown alleviated the effect of cantharidin on . . .
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