Knock-out of the five lysyl-oxidase family genes enables identification of lysyl-oxidase pro-enzyme regulated genes

HIGHLIGHTS

• who: Tatyana Liburkin-Dan and colleagues from the The NSPI-CMV-MCS-myc-His lentiviral expression vector was previously described [3]The pSpCas9(BB)-, GFP (PX458) plasmid was from Addgene (Watertown, MA, USA) (deposited by Feng Zhang [49]). The pENTR, GFP-, (#19364) plasmid was from Addgene (Watertown, MA, USA) (deposited by Eric Campeau [50]). The pLenti6/V5-DEST plasmids was purchased from Invitrogen (Thermo Fisher Scientific corporation, Waltham, Massachusetts, USA). The u2206NRF (pCMV dR, .74) and pMD2-VSV-G vectors for lentivirus production were kindly provided by Dr. Tal Kafri (University of North Carolina at Chapel Hill . . .
  

   

  

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