Stathmin 1/2-triggered microtubule loss mediates golgi fragmentation in mutant sod1 motor neurons

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  • who: Sarah Bellouze from the Primary and secondary antibodies are listed in Additional file, : Table S Other reagents were from the following suppliers: PBS, Hbss, trypsin, culture media and supplements (Invitrogen, Carlsbad, CA), taxol, nocodazole (Sigma), OCT (Thermo Scientific, Runcorn, UK), Vectashield (Vector laboratories, Burlingame, CA), Complete protease inhibitors (Roche, Basel, Switzerland), Ketamine (Bayer, Leverkusen, Germany) and Xylazine (Mérial, Lyon, France), coverslips and Superfrost Plus glass slides (Menzel, Schwerte, Germany). Plasmid expression vectors contained the following cDNAs: GFP-tagged Mannosidase-II (Dr. M. Bornens, Institut Curie, Paris, France), untagged human SOD1wt, SOD G R or SOD . . .

     

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